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Abstract
Identification of major HLA haplotypes in kindred of Pumwani sex workers in Nairobi, Kenya
T. Bielawny1, J. Kimani2, B. Ball3, J. Huntington1, S. Thavaneswaran1, S. Ramdahin3, M. Luo4, F. Plummer5
Background: A group of women in the Pumwani Sex Worker Cohort are resistant to HIV infection despite highly exposed to HIV-1 and the resistant phenotype was clustered in families. To further study investigate the genetic basis of resistance, a kindred cohort consisting of relatives of HIV resistant and susceptible women was established. The HLA class I and class II haplotypes were analyzed to confirm their genetic relatedness and to identity haplotypes and genotypes that are associated with families of HIV resistant or HIV positive women. The genetically related families will be used to identify genes that are implicated to HIV resistance and susceptibility.
Methods: DNA was isolated from PBL’s. HLA class I and II genes were typed using a TBSA method. Progeny 5 was used to assist the assignment of HLA class I and class II haplotypes. The HIV incidence of genetically related relatives and the haplotypes were analyzed using SPSS 11.0.1.
Results: Among the 171 distinct haplotypes identified from 182 individuals (91 from each group), 39 haplotypes were only found in resistant group and 38 were only found in the HIV positive group. Five haplotypes were significantly associated with the resistant group: Haplotype 3 (P= 0.006), Haplotype 4 (P=0.041), Haplotype 16(P=0.041), Haplotype 23 (P= 0.041), Haplotype 24 (P= 0.041). Three haplotypes were significantly associated with the positive group: Haplotype 30 (P= 0.014), Haplotype 34 (P=0.045), Haplotype 38(P=0.014). HIV resistant women and their genetically related families are 21.3 times more likely to be HIV-1 negative than the HIV positive women and their genetically related relatives (P=3.1 x 10-13).
Conclusions: We have identified distinct haplotypes that exist in a subgroup of HIV resistant women and their relatives. A larger study is underway to further investigate HLA haplotypes associated with resistance and susceptibility to HIV-1 infection.
AIDS 2006 - XVI International AIDS Conference
Abstract no.
CDA0018
Suggested Citation
"T.Bielawny, et al.
Identification of major HLA haplotypes in kindred of Pumwani sex workers in Nairobi, Kenya.
:
AIDS 2006 - XVI International AIDS Conference:
Abstract no.
CDA0018"
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