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Abstract
Differing Results of a Rapid HIV Testing Algorithm in Couples’ Testing Centers in Two African Cities with Different Clades of HIV
Glass S.1, Rwanda Zambia HIV Research Group Z.E.H.R.P.2
Introduction: To evaluate the results of a rapid HIV testing algorithm based on a combination of Abbott Determine HIV-1/2, Trinity Biotech Capillus HIV-1/HIV-2, and Trinity Biotech Uni-Gold HIV in Kigali, Rwanda (predominately clade A) and Lusaka, Zambia (clade C). Among HIV discordant couples, the likelihood of early infection is high in the uninfected partner, necessitating a more sensitive testing algorithm.
Methods: From August 2004 – February 2005 couples were screened with Determine, and those with both partners Determine negative were not further tested. Couples with one or both partners positive or indeterminate with Determine were tested with Capillus and Uni-Gold. Results for each test were interpreted as negative (-), positive (+), or indeterminate (I). Results given were based on >=2 tests with the same result.
Results: In Rwanda, 80% of the 4637 couples tested had two Determine (-) partners. Of 1820 samples tested further: 57.0% were (+) and 33.6% were (-) with all three rapid tests. Of the remaining 170 samples with indeterminate or discrepant results, 68% were Determine (+) or (I) and Capillus and Uni-Gold (-) and 23% were a combination of discrepant test results including 21 clients counseled as (-) and 18 as (I). In Zambia, 43.6% of the 686 couples tested were two Determine (-) partners. Of the 772 samples tested further: 80.7 % were (+) and 17.6% were (-) with all three rapid tests. Only 1.7% of samples had discrepant or (I) test results: 7 were counseled as (-), 5 as (I) and 3 as (+)
Conclusions: A third ‘tiebreaker’ rapid test allowed definitive same-day results to be provided to >99% of clients and reduced the number of indeterminate results given to 0.20% and 0.36% of clients in Rwanda and Zambia respectively. More discrepant results were seen between tests in Rwanda, where clade A predominates.
The 3rd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
MoPe15.2C19
Suggested Citation
"GlassS., et al.
Differing Results of a Rapid HIV Testing Algorithm in Couples’ Testing Centers in Two African Cities with Different Clades of HIV .
Poster Exhibition:
The 3rd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
MoPe15.2C19"
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