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Abstract
Influence of highly active antiretroviral therapy with nelfinavir on endothelial cell-derived microparticles in HIV-1-infected patients
Korzh O.1
Introduction: Endothelial cells, platelets, and oxidized low density lipoprotein (LDL) could play very important roles in the development of vascular injury in HIV-1-infected patients during highly active antiretroviral therapy (HAART). Although HIV protease inhibitors have been successfully used against HIV infection, many metabolic side effects and premature cardiovascular diseases are often associated with this therapy. The mechanisms of these complications are not clear. The aim of this study was to investigate the effect of the HAART containing nelfinavir on plasma endothelial cell-derived microparticles (EDMP) in HIV-infected patients.
Methods: We studied 79 HIV-1-infected patients receiving HAART containing nelfinavir and 79 age- and sex-matched healthy controls by measuring their levels of plasma EDMP, platelet-derived microparticles (PDMP), platelet-P-selectin (plt-PS), soluble CD40 ligand (sCD40L), and anti-oxidized LDL antibody.
Results: The concentrations of EDMP, PDMP, plt-PS, and sCD40L in HIV-1-infected patients receiving HAART containing nelfinavir were significantly higher than those in normal subjects. Levels of anti-oxidized LDL antibody were also higher in HIV-1-infected patients than in control subjects. In addition, anti-oxidized LDL antibody levels correlated with EDMP, PDMP, plt-PS, and sCD40L levels in HIV-infected patients receiving HAART. Oxidized LDL increased expression of plt-PS, and also promoted shedding of PDMP. On the other hand, activated platelets and PDMP promoted endothelial cells and THP-1 (monocytic cell line) interaction, and membrane vesiculation occurred in the presence of oxidized LDL.
Conclusions: These findings suggest that activated platelets and oxidized LDL induce EDMP generation, and that elevated EDMPs may be a sign of vascular injury in HIV-1-infected patients receiving HAART containing nelfinavir.
The 3rd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
TuPe2.3C04
Suggested Citation
" Korzh O.
Influence of highly active antiretroviral therapy with nelfinavir on endothelial cell-derived microparticles in HIV-1-infected patients.
Poster Exhibition:
The 3rd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
TuPe2.3C04"
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