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Abstract
ISOLATION OF SIVMND-2 FROM DOMESTICATED MANDRILLUS SPHINX OF SOUTHERN CAMEROON
C. Ngansop1, N. Ndembi1-2, H. Ichimura1,P. Kenmogne1, L. Kaptue1
1Faculty of Medicine and Biomedical sciences, Yaounde, Cameroon, 2Kanazawa University, Dept of viral Infection and International Health, Kanazawa, Japan
Mandrillus sphinx, a large primate living in Cameroon and Gabon, and belonging to papionini tribe, was reported to be infected with a simian immunodeficiency Virus ( SIVmndGB1). Recently we have reported a novel highly divergent SIVmnd virtually different to SIVmnd-1 and designated SIVmnd-2.To determine the prevalence of SIVmnd-2, we carried out a survey on domesticated Mandrillus sphinx in southern Cameroon.
From March to August 2001,blood was drawn from 28 nonhuman primates : 13 Mandrillus sphinx, 5 Cercopithecus nictitans and 10 Pan troglodytes troglodytes were kept as household pet in southern Cameroon.DNA of sero-reactive samples was isolated from whole blood or PBMCs. The PCR was carried out on pol-integrase region (approximately 288 bp). Amplified DNA was cloned and purified, then sequenced on both strands using ABI 310 sequencer. Newly derived SIVmnd-2 nucleotides were aligned with reference sequences from Los Alamos HIV/SIV using Clustal W with minor adjustment for protein sequences. A phylogenetic tree was constructed by the neighbor-joining method.Three seropositive Mandrillus sphinx 3/13 ( 23.1% ) were confirmed by western blotting analysis ,with New Lav BLot HIV-1 and HIV-2. Phylogenetical analysis showed that these strains clustered significantly in pol-integrase with SIVmnd-2 reference sequence ( bootsrap >70%). In an epidemiological survey of human immunodefieciency Virus (HIV) a case of a human infected with a virus serologically related to SIVmnd has been reported from Cameroon, suggesting that mandrills may represent a viral reservoir for human.To confirm thus, hypothesis further sero- epidemiological survey of human samples using SIVmnd peptide would be needed.
The 2nd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
291
Suggested Citation
" C. Ngansop , et al.
ISOLATION OF SIVMND-2 FROM DOMESTICATED MANDRILLUS SPHINX OF SOUTHERN CAMEROON.
Poster:
The 2nd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
291"
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