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Abstract
A COST EFFECTIVE ALTERNATIVE VIRAL LOAD ASSAY FOR AFRICA: CORRELATION OF CAVIDI EXAVIR LOAD QUANTITATIVE HIV-RT KIT WITH ROCHE AMPLICOR MONITOR VERSION 1.5
N. Rekhviashvili, G. Stevens, G. Corrigan, L. Scott, W. Stevens
Department of Molecular Medicine and Haematology, University of Witwatersrand, Medical School and NHLS, Johannesburg, South Africa
The Actuarial Society of South Africa estimated the number of people infected with HIV in the country at 5.3 million as at the middle of 2000. This data highlights the importance of finding more cost effective and simple alternative assays for detection and monitoring of HIV-1 viral load. The existing gold standards are still simply unaffordable for Africa. Our study evaluates the use of a new, improved ExaVir Load kit (Cavidi) for HIV-1 quantitation in a resource poor setting. The methodology is based on detection of RT (Reverse Transcriptase) enzyme activity of HIV-1, which directly correlates to the amount of free virus in plasma. Results obtained with ExaVir Load kit for 124 patients were compared to Roche Amplicor Monitor assay (v.1.5). Statistical data analysis shows good correlation between both assays (r=0.862) and R2 = 0.743 indicates that approximately 74% of the data is presented by linear regression. Data analysis for patients on HAART with serial viral load measurement demonstrated a trend and a good correlation for both assays. Based on these results we conclude that the ExaVir Load quantitative HIV-RT kit could be used as an alternative test for HIV-1 RNA quantitation.
The 2nd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
476
Suggested Citation
" N. Rekhviashvili, et al.
A COST EFFECTIVE ALTERNATIVE VIRAL LOAD ASSAY FOR AFRICA: CORRELATION OF CAVIDI EXAVIR LOAD QUANTITATIVE HIV-RT KIT WITH ROCHE AMPLICOR MONITOR VERSION 1.5.
Poster:
The 2nd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
476"
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