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Abstract
AFFORDABLE HIV-1 MONITORING: A DIRECT COMPARISON OF AN IMPROVED P24 ANTIGEN ASSAY AND THE ROCHE AMPLICOR MONITOR ASSAY
G. Stevens, N. Rekviashvili, L. Scott, W. Stevens
Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School and the NHLS, South Africa
South Africa has one of the fastest growing human immunodeficiency virus (HIV) epidemics and the largest number of people living with HIV/AIDS in the world. HIV/AIDS is the most important challenge to the economic development of Southern Africa. Measuring HIV-1 RNA load in plasma has now become a critical marker for predicting and monitoring disease progression for people on HAART. Although viral RNA determinations are indispensable for the management of HIV infection, the high cost and technical complexity of the currently available assays preclude their use in a resource poor setting. A simple, inexpensive HIV-1 quantitation kit based on modifications of the p24 antigen assay which includes a heat-denaturation and amplification boosted step (UPTA) has been evaluated in Subtype C virus. Statistical data analysis (SAS Enterprise Guide V.3.0) shows good correlation between both assays (r = 0.726) and 53% of the data is represented by linear regression (R2=0.528). When analysing patients on HAART with serial HIV-1 measurements both assays show a trend and good correlation (r = 0.7). The data generated suggests that the p24 antigen assay could be used as an alternative to the RNA viral load assay for monitoring patients on HAART.
The 2nd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
475
Suggested Citation
" G. Stevens, et al.
AFFORDABLE HIV-1 MONITORING: A DIRECT COMPARISON OF AN IMPROVED P24 ANTIGEN ASSAY AND THE ROCHE AMPLICOR MONITOR ASSAY.
Poster:
The 2nd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
475"
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