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Abstract
RELATION BETWEEN MYCOBACTERIUM AVIUM COLONIZATION, SPECIFIC CELLULAR IMMUNE RESPONSE AND VIRULENT DIFFERENCES IN HIV-1 POSITIVE PATIENTS
A.C.C. Santiago1, I.Jr. Neves2, M.C.C. Lima1, M.B. Goulart1, M.C. Lourenço2, V.C. Rolla3, M.G. Bonecini-Almeida1
1Immunology, 2Bacteriology and, 3Infectious Disease Service, Evandro Chagas Clinical Research Institut, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil
M. avium infection (MAC) is recognized as a common opportunist disease in aids patients mainly when CD4+ T cells are bellowing 100 cells/mm3 and it may be related to virulence differences in these strains. Our main goals were to evaluate the cellular immune response in aids patients who had previous MAC colonization against MAC-Isolates Clinical (IC) antigens, and using this MAC-IC to analyze virulence differences related to cytokine production, apoptosis induction on infected macrophages and resistance to intermediated nitrogen radical (nitrite oxide). The immune response induced by MAC-IC was directly correlated with the absolute number of CD4+T cells. None of them responded to their own strain when CD4+T cells were lower than 100 cells/mm3. All MAC-IC induced macrophage apoptosis after 48 hours of infection using 1:1 and 1:10 mycobateria/macrophage ratio. Regarding 1:1 ratio the apoptosis ranged from 3.1¦1.3 (mean ( SD) to 9.6¦4.7 % after 48 hours of infection with MAC-IC obtained from MAC colonized patients and 9.7¦1.6 % after 48 hours nduced by one MAC-IC obtained from sterile site. Using 1:10 ratio the apoptosis ranged from 4.7¦0.5 a 5.9¦0.1% after 48 hours of with MAC-IC obtained from MAC colonized patients and 4.5¦1.2 % after 48 induced by one MAC-IC obtained from sterile site. The number of viable bacilli reduced 6 to 12% when MAC-IC was exposed to sodium nitrite. MAC-CI obtained from colonized patients showed a tendency to induce greater production of all cytokines (IL-6, 8, 10, 12 and TNF-() compared to MAC-CI obtained from sterile site, without statistical difference. However, two MAC-IC obtained from colonized patients induced greater TNF-( and IL-10 production compared into this group. The differential apoptosis and cytokine induction by MAC-IC could be related to so call virulence differences, since both varied among MAC-IC and may be used as possible tools to study strain differences not detected by classical microbiological or clinical analysis.
The 2nd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
899
Suggested Citation
" A.C.C. Santiago , et al.
RELATION BETWEEN MYCOBACTERIUM AVIUM COLONIZATION, SPECIFIC CELLULAR IMMUNE RESPONSE AND VIRULENT DIFFERENCES IN HIV-1 POSITIVE PATIENTS.
Poster:
The 2nd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
899"
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