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Abstract
BIOCHEMICAL ASSESSMENT OF LIPID METABOLISM AND HEPATOCELLULAR FUNCTIONS IN RITONAVIR-NELFINAVIR MICE ON DIETS SUPPLEMENTED WITH DIETARY FIBRES AND OXIDANTS
B.A. Iwalokun
Biochemistry Dept, Lagos State University, PMB, 1087, Apapa, Lagos, Nigeria
There is a growing public health concern on the use of protease inhibitors in HAART following several demonstrations of their ability to induce hyperlipidaemia, exacerbate lipid dysfunction and cause liver abnormalities.
However, biochemical evidence concerning the clinical benefits of dietary fibres with or without vitamins and mineral supplementation in PI based treatment regimens is grossly inadequate.
In this study, 96 healthy mice were randomized equally into two treatment groups and each group was subdivided into three groups of 16 mice each: 1. Control (Basal diet); 2. High fibre diet; 3. High fibre diet supplemented with zinc and vitamins A, C and E. The mice were orally given 30mg/kg body weight of neflinavir or ritonavir for 28 days. Serum levels of triglyceride, cholesterol, HDL-C, alanine transaminase (SGPT), glutamate transaminase (SGOT) and alkaline phosphatase were then determined colorimetrically.
In the two PI treated groups, 50 – 70% increase in HDL-C and 30 – 85% reduction in the serum levels of other lipid parameters and enzyme activity were observed in mice in groups 2 and 3 when compared to the control. Analysis of data further showed that the zinc, vitamins and fibre-enriched diet compared with fibre alone, elicited improvement in the lipid profile and enzyme activities including cholesterol (2.48 + 0.25 vs. 3.04 + 0.23mM; P < 0.05) and SGOT (16.3 + 0.4 vs. 17.3 + 0.6U/L; P < 0.05).
The present results reveal the clinical benefits inherent in the use of dietary fibres, zinc and vitamins as an adjunct in PI based anti-retroviral interventions.
The 2nd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
713
Suggested Citation
" B.A. Iwalokun, et al.
BIOCHEMICAL ASSESSMENT OF LIPID METABOLISM AND HEPATOCELLULAR FUNCTIONS IN RITONAVIR-NELFINAVIR MICE ON DIETS SUPPLEMENTED WITH DIETARY FIBRES AND OXIDANTS.
Poster:
The 2nd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
713"
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