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Abstract
PRESENCE OF KEY RESISTANCE MUTATIONS IN CRF02-AG RECOMBINANT STRAINS OF UNTREATED HIV INFECTED PATIENTS FROM ABIDJAN (CÔTE D’IVOIRE)
T.T. D’Aquin1-5, P. Recordon-Pinson5, A. Minga2, D. Ekouevi2, M. Faure5, M.H. Schrive5, D. Bonard1-2-3, R. Salamon4, B. Masquelier5, E.J. Fleury5
1CIRBA, Abidjan, Côte d'Ivoire, 2PAC-CI, Abidjan, Côte d'Ivoire, 3CeDRes, Abidjan, Côte d'Ivoire, 4Inserm U330, Bordeaux, France, 5Université de Bordeaux 2, Bordeaux, France
Objectives : The aim of the study was to document the epidemiology of the primary resistance mutations before antiretroviral (ARV) therapy in C¶te d’Ivoire and to assess the genetic polymorphism of HIV-1 strains circulating in the population.
Methods: Untreated patients from Abidjan, C¶te d'Ivoire, enrolled in a cohort of regular blood donors (PRIMO-CI) and in a trial of efficacy of AZT/NVP for the prevention of mother to child transmission of HIV (DITRAME PLUS) were included in 2001 and 2002 in the study. Reverse transcriptase (RT), protease (PR) and env genes were submitted to sequence analysis and phylogenetic bootstrap was processed for the three genes.
Results: 48 patients from PRIMO-CI and 59 patients from DITRAME PLUS were included. The mean age was 28 years and they were contaminated through heterosexual contact. The mean TCD4 count was 458/(l and the median was 395/(l (range: 78-1120). 90/107 HIV-1 isolates clustered with CRF02_AG; others subtypes were found : A, CRF01_AE and intersubtype recombinants, in 5, 1 and 11 patients respectively. Non nucleoside reverse transcriptase inhibitors (NNRTI) resistance mutations were present in four patients : K101E (one patient), K103N (two patients), P236L (one patient). One NRTI resistance mutation (K219Q) was noticed in one patient. A key protease inhibitor (PI) resistance mutation (N88D) was detected in one patient. Other substitutions, V106I, I135T and V245Q associated to low level resistance to NNRTI were observed. The M36I mutation, a minor PI resistance mutation, was retrieved in 94% of CRF02_AG strains.
Conclusions: In a background of non-B (mainly CRF02) polymorphism, we could detect ARV resistance mutations in na´ve patients from Abidjan. These data revealed that there is circulation and transmission of HIV-1 resistant strains and suggest the necessity to establish a longitudinal survey in this country.
The 2nd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
781
Suggested Citation
" T.T. D̻Aquin -5, et al.
PRESENCE OF KEY RESISTANCE MUTATIONS IN CRF02-AG RECOMBINANT STRAINS OF UNTREATED HIV INFECTED PATIENTS FROM ABIDJAN (CÔTE D’IVOIRE).
Poster:
The 2nd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
781"
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