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Abstract
HIV-1 SUBTYPE B POLYMORPHISM IN SOUTH AMERICA: CHARACTERIZATION OF PROVIRAL DNA ISOLATES FROM VENEZUELA
E. Castro1, M. Moreno1, L. Deibis1, S. Salmen2, L. Berrueta 2, G. Echeverría de Pérez1
1Immunology Institute, Central University of Venezuela, Caracas, 2Clinical Immunology Institute, Merida, Venezuela
Background: Data of HIV-1 group M subtypes available for South American countries(SAC) have mainly demonstrate the prevalence of Subtype B(~80%) followed by Subtype F(~8%) and the emerging identification of CRF_012 B/F. However, besides subtype B env variant from Brazil, very little is known about subtype B polymorphism within SAC. Therefore we studied HIV+ individuals coming from geographical regions with different migration patterns in Venezuela: Andean Region(AR),Central Region(CR) and Other Regions(OR).
Methods: Proviral DNA isolated from outpatients with confirmed HIV infection was amplified by nested PCR using primers sets ED5/12; ED31/33 for env region and H1G777/H1P202, g17/H1Gag1584 for gag gen. Heteroduplex Mobility Assay(HMA) for env and gag regions respectively, was performed. Chi square test was used for statistical comparisons.
Results: A total of 104 individuals(f= 22; m= 82) coming from the AR(n= 44), CR(n= 42) and OR(n= 18) of the country volunteered for the study. A total of 87% were 15 to 44 years old. Sexual transmission route was verified in 96.15% of the study group. According to HMA results a high prevalence of subtype B was verified for gag region(98%) and env region(92%) respectively. The remaining samples showed indeterminate patterns and were confirmed also as subtype B by env/gag nucleotide sequencing. Based on the comparison of a migration distance score of heteros formed with B subsubtypes vs. heteros formed with subsubtypes of other subtype references, we defined subtype B patterns as “Clear or Polymorphic “. When comparing subtype B migration patterns between CR and AR isolates, statistically significant higher polymorphism in gag region (p =0,01508177) was found for AR samples and a higher proportion of polymorphism in env region was observed among CR samples.
Conclusions: Our results suggest that genomic polymorphism differences among subtype B strains from Venezuela can be related to geographical regions. Thus, we can not determine the meaning of this polymorphism in SAC, it seems that gag/env HMA can be also an appropriate tool to compare HIV-1 subtype B strains within subepidemics of South America where nucleotide sequencing is not widely available for molecular surveillance and vaccine strategies move on to clinical trails.
The 2nd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
235
Suggested Citation
" E. Castro , et al.
HIV-1 SUBTYPE B POLYMORPHISM IN SOUTH AMERICA: CHARACTERIZATION OF PROVIRAL DNA ISOLATES FROM VENEZUELA.
Poster:
The 2nd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
235"
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