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Abstract
THE STUDY OF GLYCYRRHIZIC ACID BINDING SITES BY PHAGE DISPLAY METHOD
T.V. Ilina, N.V. Fedyuk, O.U. Tumanova, V.N. Kuvshinov, A.A. Ilychev, A.G. Pokrovsky
State Research Center Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk Region, Russia
Glycyrrhizic acid – is the most important component of a Liquorice root, having a wide range of biological activities, such as anti-inflammatory, anti-viral, hypolipidemic, anti-oxidative and interferon-inducing. One of the most interesting for virologists features of glycyrrhizic acid (GA) is an ability to prevent reproduction of a many viruses, particularly HIV-1. One of the supposed mechanisms of GA action is an inhibition of protein kinases activity.
The main purpose of our work was detecting all probable GA binding sites for understanding a wide spectrum of its activity. Also, it may be important for future drug development and can avoid occurrence of side reaction. For this we used the technology of phage display, which allows the identified functional protein domains and peptide ligands for different receptors. Using methods of affinity selection and ELISA, we selected phages with peptides specifically interacting with GA.
The aminoacids sequences of selected peptides don’t have strong homology with each other, but they all have same features. Comparison of selected peptides with sequences data bases SWISS-PROT and TrEMBL exhibit a presence of high homology with tyrosine proteinkinases, serine-threonine proteinkinases, tyrosine phosphatase and some others proteins. The results of searching virus proteins homology using BLAST programs detected that glycyrrhizic acid have affinity to the many surface proteins of such well-known human viruses, as HIV-1, Herpesviruses and Hepatitis C. It was demonstrated that glycyrrhizic acid could bind directly with gp 120 proteins and prevent virus attachment with a CD4 receptor.
The 2nd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
872
Suggested Citation
" T.V. Ilina, et al.
THE STUDY OF GLYCYRRHIZIC ACID BINDING SITES BY PHAGE DISPLAY METHOD.
Poster:
The 2nd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
872"
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