|
|
Abstract
PARTIAL RESTAURATION ON HIV-SPECIFIC CD4 T CELL RESPONSES WITH DENDRITIC CELLS.
GRASSI F, CARCELAIN G, BONDUELLE O, ALATRAKCHI N, VALANTIN M, KATLAMA C, AUTRAN B
Background: we reported a defect in CD11c+ dendritic cells (DC) producing IL-12 that correlated with the HIV load in HIV-infected patients, even treated. We now investigated whether in vitro addition of DC or IL-12 could correct the defects in the CD4 T cell responses to HIV for future immune-based therapies. Methods: PBMC from 44 HIV-infected donors (24 untreated: 12 Slow Progressors (SP), 12 Progressors (P) and 24 treated patients (ART) were assayed in MLR or proliferative assays to HIV-p24 with addition of autologous DC derived from monocytes (MDDC) or addition of IL-12. Frequencies of HIV-specific CD4 T cells were evaluated by ELISPOT. Results: the MDDC efficacy in MLR was decreased in progressors, even treated, compared to SP. The MDDC addition as well as the IL-12 one restored the HIV-specific memory CD4 T cell response (mediated by CD45RO+ cells) in SP, but only marginally in P and ART. The median frequencies of HIV-specific CD4 T cells were 550 and 35/106 PBMC in SP and P, respectively. Conclusion: addition of DC and IL-12 similarly enhances the HIV-specific memory CD4 Th responses in slow progressors but not in progressors even when treated, suggesting that defects in APCs might play a role in, but cannot compensate, the quantitative defects of HIV-specific CD4 Th1 cells.
The 1st. IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
79
Suggested Citation
"GRASSIF, et al.
PARTIAL RESTAURATION ON HIV-SPECIFIC CD4 T CELL RESPONSES WITH DENDRITIC CELLS..
Oral Presentation:
The 1st. IAS Conference on HIV Pathogenesis and Treatment
:
Abstract no.
79"
|
|
|