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Abstract
Relationship between oxidative stress, endothelial injury and coagulation activation in HIV-infected patients receiving highly active antiretroviral therapy
Korzh O.1
Introduction: The aim of the present study was to establish whether enhanced oxidative stress (OS), involving endothelial injury, activation of coagulation, and inflammatory reaction, could be implicated in HIV-infected patients during highly active antiretroviral therapy (HAART).
Methods: We studied 126 HIV-infected, HAART-treated men and 112 healthy male controls matched for age and body mass index. Markers of OS, endothelial injury, coagulation, and cytokines, were measured in the plasma of patients and of healthy controls by ELISA methods. Remodeling of the carotid arteries was assessed by measuring the intima-media thickness (IMT) as a surrogate of atherosclerotic disease in all groups. To evaluate possible influence of acute phase reaction, reinvestigation was performed after 6 months.
Results: Markers of OS, endothelial injury, and extrinsic coagulation pathway activation were significantly elevated in HIV-infected patients during HAART. The von Willebrand factor antigen (vWF:Ag) levels were more increased in HIV-infected patients than in control group. Furthermore, the plasma levels of tumour necrosis factor alpha, monocyte chemo-attractant protein 1, and macrophage inflammatory protein 1 beta were significantly higher in HIV-infected patients when compared with the controls. The IMT was strongly and directly correlated with Cu/Zn superoxide dismutase. Both IMT and Cu/Zn superoxide dismutase were positively correlated with age, thrombomodulin, vWF:Ag, tissue factor, tissue factor pathway inhibitor, monocyte chemoattractant protein 1, macrophage inflammatory protein 1 beta, and tumour necrosis factor alpha levels. Multivariate analysis identified vWF:Ag as the only independent variable significantly associated with an increased IMT.
Conclusions: The present study suggests that enhanced OS, involved pro-atherogenic cytokine and chemokines levels, endothelial injury, and coagulation activation may constitute a pathway for progression of atherosclerosis in HIV-infected patients receiving HAART. The significant, independent association between IMT and vWF:Ag should be assessed in future studies to determine whether vWF:Ag elevation is causative or a by-product of the increased IMT.
The 3rd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
TuPe2.3C01
Suggested Citation
" Korzh O.
Relationship between oxidative stress, endothelial injury and coagulation activation in HIV-infected patients receiving highly active antiretroviral therapy.
Poster Exhibition:
The 3rd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
TuPe2.3C01"
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