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Abstract
Evaluation of ESAT-6 и CFP-10 – specific T lymphocytes in co-infected with HIV-1 and M. tuberculosis Bulgarian patients during specific anti-tuberculosis therapy
Markova R.1, Drenska R.1, Terzieva V.1, Todorova Y.1, Elenkov I.2, Yankova M.2, Yankova M.2, Kostov K.2, Stefanova D.3
Introduction: Monitoring the efficacy of anti-tuberculosis therapy is crucial both for the individual patient and for better control of the spread of Tuberculosis. Certain highly immunogenic secreted antigens of M. tuberculosis such as ESAT-6 and CFP-10, offer hope for more specific diagnosis of M. tuberculosis infection. Our aim was to study the dynamics of ESAT-6 and CFP-10 – specific IFN-gamma producing T cells during specific anti-tuberculosis therapy in co-infected with HIV-1 and M. tuberculosis patients.
Methods: 10 HIV/TB patients were studied at the time of diagnosis and 3 months after they started therapy. Eight of them were with microbiologically confirmed tuberculosis. An in vitro enzyme-linked immunospot (T SPOT-TB, Oxford Immunotec) assay and an ELISA (QuantiFERON-TB Gold, Cellestis, Australia) that detect T cell-mediated IFN-gamma responses to ESAT-6 and CFP-10 proteins were used.
Results: All patients had positive results at diagnosis; 3 months later the responses to ESAT-6 and CFP-10 were still detectable in two patients with microbiological isolation and/or absence of clinical improvement after treatment.
Conclusions: On the basis of our data obtained even from a small number of patients we conclude that the in vitro ELISPOT and ELISA assays are useful tools in monitoring the efficacy of antituberculosis therapy especially in HIV/MTB patients.
Acknowledgements: We are grateful to Ajit Lalvani, Chris Granger (Oxford Immunotec) and Jim Rothel (Cellestis) for their scientific collaboration.
This study was supported by Grant L-1101/01 of the National Fund for Scientific Research of the Ministry of Education and Science, republic of Bulgaria.
The 3rd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
TuPe7.1C02
Suggested Citation
"MarkovaR., et al.
Evaluation of ESAT-6 и CFP-10 – specific T lymphocytes in co-infected with HIV-1 and M. tuberculosis Bulgarian patients during specific anti-tuberculosis therapy.
Poster Exhibition:
The 3rd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
TuPe7.1C02"
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