|
|
Abstract
Evaluation Of A Phage Based Test For The Rapid Detection Of Mycobacterium Tuberculosis In Sputum From HIV-Positive Cases
Otive-Igbuzor E.J.1, Fagade O.1, Idigbe E.O.2, Onyejepu N.N.2, Okwelogu L.2, Dan-Okoli J.2, Onubogu C.2, Cadmus I.1
Introduction: Tuberculosis (TB) is the biggest killer of People Living with HIV/AIDS worldwide. Its rapid detection has important implications for early treatment. Conventionally, TB is diagnosed through the examination of direct sputum smears for the presence of Acid Fast Bacilli (AFB). Though microscopy has high specificity, its sensitivity is low, requiring up to 10,000 bacilli per ml of sputum to detect AFB. HIV alters immune response, reducing the number of AFB present in sputum and result in presentation of more smear negative cases. There is an urgent need for rapid, easy and affordable methods to detect smear negative tuberculosis which has been reported to be infectious.
Methods: This study was designed to evaluate the sensitivity and specificity of a phage-based test, FASTPlaque-TBTM (FPTB) (BIOTEC Laboratories, Ipswich, UK,)using phage amplification technology for the detection of Mycobacterium tuberculosis in sputum specimens from both HIV positive and negative cases
Results: The results demonstrate high sensitivity (96%) and specificity (93%) of FPTB and the ability to detect a significant number (95%) of smear negative cases.
Conclusions: The FASTPlaque-TB Assay has a strong potential to improve the diagnosis of TB in developing countries where HIV/AIDS has assumed epidemic proportions.
The 3rd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
TuPe15.3C01
Suggested Citation
"Otive-IgbuzorE.J., et al.
Evaluation Of A Phage Based Test For The Rapid Detection Of Mycobacterium Tuberculosis In Sputum From HIV-Positive Cases .
Poster Exhibition:
The 3rd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
TuPe15.3C01"
|
|
|