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Abstract
Characterization of mutations in HIV type 1 isolates from 135 Cambodian pregnant women and recently infected patients, naive to antiretroviral drugs
Ly N.1, Recordon-Pinson P.2, Phoung V.3, Kruy Leang S.4, Koum K.5, Chhum V.6, Glaziou P.1, Fleury H.2, Reynes J.M.1
Introduction: The study aims: (i) To sequence HIV-1 from plasmas of infected Cambodian ARV naive patients and determine the polymorphism of three viral genes, namely protease (Prot), reverse transcriptase (RT) and envelope (Env); (ii) To observe potential mutations involved in resistance to ARV (PI, NRTI, NNRTI) and determine their prevalence.
Methods: Two ARV naive populations have been studied: one group of HIV-1 individuals infected for less than one year and a second group of pregnant women having been informed of their seropositivity for less than one year. Viral RNA was extracted from plasma, retrotranscribed and amplified in Prot, RT and Env C2V3 before sequencing. Phylogenetic trees were constructed on the three genes using ClustalX software; polymorphism of Prot, RT and Env was noted; interpretation of drug resistance mutations was conducted on Prot and RT according to ANRS 2004 algorithm.
Results: One hundred thirty five cases were analyzed (20 recently infected patients and 115 pregnant women). CRF01_AE was predominant (129 cases) followed by subtype B (2 cases), CRF15_01B (2 cases) and unknown types (2 cases). Polymorphism substitutions (>50%) of CRF01_AE at drug resistance positions were only in Prot were : E35D (87%), M36I (100%), R41K (96%), H69K (100%). Two strains bearing major resistance mutations at position N88D and M46I to PI Nelfinavir and Indinavir respectively and four strains bearing resistance mutations to NRTI (V75M, L215I) and one to NNRTI (K101E) were observed leading to an estimated global prevalence of resistance at 5.2% in the sample studied.
Conclusions: This is the first study on HIV-1 drug resistance in ARV naive patients of Cambodia. Within a predominant of CRF01_AE, the prevalence of viral strains bearing drug resistance mutations seems to be low. Extension of available ARV treatments will require follow up studies in the next future.
The 3rd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
WePe4.1C01
Suggested Citation
"LyN., et al.
Characterization of mutations in HIV type 1 isolates from 135 Cambodian pregnant women and recently infected patients, naive to antiretroviral drugs.
Poster Exhibition:
The 3rd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
WePe4.1C01"
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