|
|
Abstract
Highly conserved HIV-1 subtype B strains circulating in Honduras
Castro E.1, Lorenzana I.2, Moreno M.3, Echeverria G.3
Introduction: : HIV/AIDS is the second leading cause of death in Honduras.Previous studies from Honduras have shown only subtype B strains when analyzing envelope (env) and LTR genes. Presently, we are showing phylogenetic data derived from env, gag and pol genes of HIV isolates of 3 individuals from Honduras with HAART failure, compared to other subtype B strains.
Methods: Proviral DNA was amplified by nested PCR. Nucleotide sequencing was performed in all PCR derived amplicons using the Big Dye Terminator Kit (ABI PRISM 377). Resistance mutation analysis of pol sequences was obtain with the online Stanford University website database. Blast subtyping, sequence alignments with ClustalX and neighbor-joining trees with reference sequences using a bootstrap value of 1000 were constructed. Additionally, amino acid alignments with HXB2 and subtype B strains from Venezuela were analyzed.
Results: Phylogenetic analysis of all env, gag and pol sequences verified subtype B with high bootstrap values. Also no breakpoints with other subtypes were observed when blasting env, gag and pol nucleotide sequences to reference sequences (http://www.ncbi.nih.gov). V3 loop tip motifs corresponded to GPGR (2/3) and GPGQ (1/3). A positive selection due to non-synonymous substitutions was found for C3 alignment of sequences from Honduras. Amino acid alignment of gag sequences with HXB2 reference strain revealed a constant 9-mers motif “HSGHKTRT”, whereas no motif was shared with gag sequences from Venezuela. Resistant mutations to RT and protease inhibitors where found in correspondence to patients HAART history.
Conclusions: Despite the small number of sequences analyzed in this study, results confirmed a very conserved subtype B genomic profile of the isolates from Honduras when compared to other subtypes as well as to other B clades from HIV patients of Venezuela. Positive selection in the C3 region and constant motifs in gag gene are hints towards a population genetic based correlation to a highly conserved subtype B circulating in Honduras.
The 3rd IAS Conference on HIV Pathogenesis and Treatment
Abstract no.
MoPe14.1B01
Suggested Citation
"CastroE., et al.
Highly conserved HIV-1 subtype B strains circulating in Honduras.
Poster Exhibition:
The 3rd IAS Conference on HIV Pathogenesis and Treatment:
Abstract no.
MoPe14.1B01"
|
|
|