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Abstract
A simple, rapid analytical procedure for lamivudine (an antiretroviral agent) in tablets
Egieyeh S.1, Builders P.1, Oqua D.A.N.1, Kunle O.O.1, Obodozie O.1, Inyang U.S.1
Objective: To study the complex formed by chloranilic acid and lamivudine with a view to using it as a simple and cheaper analytical method for quantitatively assessing lamivudine in its dosage form.
Method: Equal concentrations (0.05 M) of chloranilic acid in 1, 4, Dioxane and lamivudine solutions were prepared. The absorption –spectra of these solutions were determined using a Shimadzu 160A UV-Spectrophotometer. Equal volumes of equi-molar concentrations of lamivudine and chloranilic acid were mixed and the absorption spectrum of the mixture was then determined. The molar reactivity of the reaction was determined using Job’s continuous variation method. The stability constant and the effect of light on the complex were also determined. Twenty tablets each of four commercial samples were used for quantitative analysis using this method.
Result: The Chloranilic acid and the drug solutions both gave peculiar absorption spectra with absorption maxima (λmax) at 416 nm and 295 nm respectively. Mixing the lamivudine and the chloranilic acid solutions resulted in a purple-colored complex that gave an absorption maximum at 549 nm.
The molar ratio of lamivudine to chloranilic acid employed in the charge transfer reaction was 1:1. The stability constant, K of the complex formed was 4.03x103.
The calibration curve (n=5) at 549nm revealed very small intercept (0.006206) and the good linear relationship (r=0.9989) between the absorbance and the drug concentration over the range 10-400mg/ml. The obtained mean recovery percentage values (±SD) of the labeled amounts ranged from 89.88±1.09 to 103.87±1.02.
Conclusion: The Bathochromic shift in the absorption spectra of the lamivudine-chloranilic acid complex enabled the analysis of the drug in the visible range of the absorption spectrum. The quantitative determination of lamivudine using this method proved to be highly sensitive, precise, accurate and reproducible. A simple, rapid and accurate spectrophotometric method was therefore developed for the analysis of the antiretroviral.
4th IAS Conference on HIV Pathogenesis, Treatment and Prevention
Abstract no.
WEPEA100
Suggested Citation
"EgieyehS., et al.
A simple, rapid analytical procedure for lamivudine (an antiretroviral agent) in tablets.
Poster exhibition:
4th IAS Conference on HIV Pathogenesis, Treatment and Prevention:
Abstract no.
WEPEA100"
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