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Abstract
Frequency and site mapping of HIV-1/SIVcpz, HIV-2/SIVsmm and other SIV gene sequence cleavage by various bacteria restriction enzymes: precursors for a novel HIV inhibitory product
Wayengera M.A.1, William B.2, Henry K.3
Objectives: Resistance, toxicity and virologic failure have underlined the need to develop new HIV inhibitory products. Basing on the natural bacteria “restriction modification system” antiviral immune model, we set out to analyze the effects of various restriction enzymes on the HIV genome.
Methods:
Design: A computer simulated model using Webb cutter Version 2.0, and cytogenetic analysis.
Materials: 339 restriction enzymes from promega database,10 HIV-1/SIVcpz genes, 10 HIV-2/SIVsmm genes and 10 Other SIV genes.
Interventions: Gene sequences were fed into Webb cutter 2.0 set to search enzyme with at least 6 recogition base pairs(palindromes) A Comparision invitro Cytogenetic analysis of GAG, POL and ENV genes was done after.
Results: Of the 339 enzymes used, 238[70.2%] cleaved the HIV-1/SIVcpz A1.BY.97.97BL006_AF193275 genome with 9037bps compared to 225 [66.4%] and 219[64.6%] for the HIV-2/SIVsmm A.GM.x.MCN13_AY509259 genome [9713 bps] and Other SIV B.FR.83.HXB2_LAI_IIIB_BRU_K03455 genome [9719 bps] respectively. Individual genes had differing but potent susceptibility to the enzymes, with a 98.9% PPV [95%CI, 97.2%-99.6%] for invitro cytogenetics
Conclusions: The natural bacteria RMS antiviral immune model offers precursors for developing novel HIV and other viral therapeutic Molecules.
4th IAS Conference on HIV Pathogenesis, Treatment and Prevention
Abstract no.
CDA036
Suggested Citation
"WayengeraM.A., et al.
Frequency and site mapping of HIV-1/SIVcpz, HIV-2/SIVsmm and other SIV gene sequence cleavage by various bacteria restriction enzymes: precursors for a novel HIV inhibitory product.
:
4th IAS Conference on HIV Pathogenesis, Treatment and Prevention:
Abstract no.
CDA036"
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