International AIDS Society

Level of naïve/memory CD4 T cells prior to initiation of antiretroviral therapy predicts the response to treatment

Sitdykova Y.¹, Serebrovskaya L.¹, Kravchenko A.¹, Pokrovsky V.¹

Objectives: To determine if the baseline levels of naïve/memory CD4 T cells and expression of some surface antigens affect the response to the treatment.
Methods: We measured the level of naïve/memory CD4 T cells and CD8 cells, expressing CD28 and CD57 in 45 HIV-infected treatment-naïve subjects, mean age 28+9,9. The analysis was performed using flow cytometry (Epics XL, Beckman Coulter). We determined the percent of CD4+CD45Ra+CD62L+ cells (naïve), CD4+CD45RO+ (memory), CD8+CD28+ and CD8+CD57+ cells. We calculated naïve/memory CD4 T cells ratio prior to initiation of antiretroviral therapy. The patients were divided into two groups according to the value of the ratio – group 1 was consisted of the patients with lower values of the ratio, group 2 included the patients with higher values. CD4 T cell increase was compared in both groups after 24 weeks of therapy.
Results: Patients with higher values of naïve/memory CD4 T cells ratio demonstrated significantly higher CD4 T cell increase after 24 week of treatment as it shown in the Table.

Group#	n	naïve/memory CD4 T-cells ratio	median%CD4 Week 0	median%CD4 Week 24	median CD4 cell/mm3 Week 0	median CD4 cell/mm3 Week 24	CD4 increase from baseline(%)
1	25	<0,6	19	21	281	291	3,6
2	20	³0,6	20	26	262	423	61,5

The correlation between the value of the ratio and CD4 T cell increase was found (r = 0,3141; p=0,02). We also noticed that CD4 T cell increase was more prominent in patients with higher level of CD28 expression on CD8 cells and lower level of CD8+CD57+ cells, although statistically significant correlation was not found.
Conclusions: Patients who had higher level of naïve and lower level of memory CD4 T cells, higher naïve/memory CD4 T cells ratio, lower numbers of CD8+CD57+ cells and higher numbers of CD8+CD28+ cells prior to initiation of antiretroviral therapy demonstrated stronger immunological response to treatment.

4th IAS Conference on HIV Pathogenesis, Treatment and Prevention
Abstract no. CDB077

Suggested Citation
"SitdykovaY., et al. Level of naïve/memory CD4 T cells prior to initiation of antiretroviral therapy predicts the response to treatment. : 4th IAS Conference on HIV Pathogenesis, Treatment and Prevention: Abstract no. CDB077"

Abstract

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