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Abstract
Comparison of reverse transcriptase polymorphism in Cambodian women with CRF01_AE subtype before and after single-dose nevirapine prophylaxis
Ly N.1, Phoung V.2, Min D.C.2, Srey C.3, Kruy L.S.4, Koum K.5, Chhum V.6, Glaziou P.2, Fleury H.7, Reynes J.-M.1
Objective: Our objective was to explore amino-acid changes of the reverse transcriptase (rt) of CRF01_AE isolates from pregnant women before and after single dose nevirapine (sdNVP) exposure in a setting of prevention of HIV-1 mother-to-child transmission (PMTCT) program. The molecular characterization of these isolates may provide relevant information on specific patterns of resistance related to CRF01_AE subtype and treatment regimens.
Method: Follow-up after PMTCT consisted of plasma sampling at 2, 6, and 52 weeks post-partum (pp) in women naïve to antiretroviral drugs except the PMTCT regimen. The first 250 amino-acids of the rt gene were amplified and sequenced using the conventional genotyping technology. Then, the frequencies of amino-acid and drug resistance mutation (RM) was correlated for each position with sampling time and PMTCT treatment.
Results: Repeated observations (sequences obtained at pre-NVP, week 2 pp and week 6 pp were available for 51 women: 35 received sdNVP and 16 zidovudine (ZDV) + sdNVP. No nucleoside reverse transcriptase inhibitor RM was detected. The proportion of isolates with non nucleoside reverse transcriptase inhibitors (NNRTI) RM in the group treated with sdNVP (n = 35) increased from 0% pre-NVP to 22.9% at week 2 pp and 22.9% at week 6 pp (8/35; P = 0,0078). In the group treated with Zidovudine + sdNVP (n = 16), the proportion with RM increased from 0% to 31.3% (5/16; P = 0.0625) at week 2 pp and decreased to 18.8% at week 6 pp. Most of the RM was not found at week 52 pp. Furthermore, no apparent subtype-specific treatment-related mutations were detected.
Conclusion: NNRTI RM occurrence in CRF01_AE strains is similar to that observed in subtype A, D and CRF02_AG strains after exposure to antiretroviral drugs for PMTCT. The ANRS rt RM algorithm is suitable for genotyping CRF01_AE strains in this context.
4th IAS Conference on HIV Pathogenesis, Treatment and Prevention
Abstract no.
CDB036
Suggested Citation
"LyN., et al.
Comparison of reverse transcriptase polymorphism in Cambodian women with CRF01_AE subtype before and after single-dose nevirapine prophylaxis.
:
4th IAS Conference on HIV Pathogenesis, Treatment and Prevention:
Abstract no.
CDB036"
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